Objectives The aim of the study was to detect single-strand breaks in deoxyribonucleic acid (DNA) in mononuclear blood cells of fire fighters exposed to o-nitroanisole and other substances released into the environment during an accident in a chemical plant.
Methods The level of DNA single-strand breaks in mononuclear blood cells was detected by alkaline elution.
The results were compared for 16 fire fighters who worked in a contaminated area for about 8 h and two reference groups (one of fire fighters who had not worked in the contaminated area, group I, and one of persons without any apparent occupational exposure to genotoxic substances, group II).
Results The mean normalized elution rate (nER) 19 d after the accident was slightly but statistically significantly (P<0.05) higher for the exposed fire fighters [mean 1.48±95% confidence interval (95% CI) 0.21] than for reference group I (mean 1.21±95% CI 0.21) or reference group II (mean 1.17±95% CI 0.18).
No. statistically significant difference was found between reference groups I and II.
Another analysis was performed three months after the first.
The level of DNA single-strand breaks (mean nER 1.12±95% CI 0.11) was no longer increased in comparison with the level. s of the reference groups, Conclusions DNA single-strand breaks were increased in fire fighters exposed to o-nitroanisole and other substances.
Mots-clés Pascal : Exposition professionnelle, Toxicité, Homme, DNA, Carcinogène, Mutagène, Cassure monocaténaire, Pompier, Accident, Industrie chimique, Mononucléaire, Surveillance biologique, Médecine travail, Anisole(2-nitro)
Mots-clés Pascal anglais : Occupational exposure, Toxicity, Human, DNA, Carcinogen, Mutagen, Single strand break, Firefighter, Accident, Chemical industry, Mononuclear cell, Biological monitoring, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0210791
Code Inist : 002B03L06. Création : 09/06/1995.