The persistence of hepatitis A virus (HAV) was determined both in mixtures of septic tank effluent (STE) with dairy cattle manure slurry (DCMS) and in mixtures of STE with swine manure slurry (SMS).
HAV was consistently inactivated more rapidly in the two types of mixed wastes than in STE alone or in the control Dulbecco's phosphate-buffered saline (PBS).
At 5oC, the D values (time, in days, for a 90% reduction of virus titer) were 34.6 for the mixed STE and DCMS, 48.5 for the mixed STE and SMS, 58.5 for STE, and 217.4 for the Dulbecco's PBS control.
At 22oC, the D values were 23.0,17.1,35.1, and 90.1 for the four suspension media, respectively.
A comparison of HAV inactivation in mixed wastes subjected to different treatments at the same pH and temperatures showed that the virus inactivation in the mixed wastes was related, at least in part, to microbial activity.
In mixed STE and DCMS, the D values at 25oC were 8.3 for raw mixed wastes, 15.1 for autoclaved mixed wastes, and 9.6 for bacterium-free filtrate of raw mixed wastes ; D values at 37oC were 6.8,10.1, and 7.0 for these three suspension media, respectively.
In mixed STE and SMS, the D values at 25oC were 8.1 for raw mixed wastes, 14.3 for autoclaved mixed wastes, and 9.1 for bacterium-free filtrate of raw mixed wastes ; the D values at 37oC were 6.8,9.4, and 6.9 for the three suspensions, respectively.
Mots-clés Pascal : Virus hépatite A, Fosse septique, Déchet animal, Déchet domestique, Inactivation, Microorganisme, Facteur milieu, Activité microbienne, Picornaviridae, Virus
Mots-clés Pascal anglais : Hepatitis A virus, Septic tank, Animal waste, Domestic refuse, Inactivation, Microorganism, Environmental factor, Microbial activity, Picornaviridae, Virus
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0186062
Code Inist : 002B30A02A. Création : 09/06/1995.