Objectives-To validate a provocative chelation test with DMSA by direct comparison with the standard EDTA test in the same subjects ; and to compare and contrast the predictors of lead excretion after DMSA with those after EDTA.
A provocative chelation test with DMSA could thus have wide potential application in clinical care and epidenuological studies.
Methods-34 male lead workers in the Republic of Korea were given a single oral dose of 10 mg/kg DMSA, urine was collected over the next eight to 24 hours, and urine volume and urinary lead concentration determined at 0,2,4,6,8, and 24 hours.
Either two weeks before or two weeks after the dose of DMSA 17 of these workers also received 1 g intravenous EDTA followed by an eight hour urine collection with fractionation at 0,2,4,6, and 8 hours.
Urinary lead concentration peaked at two hours after DMSA and four hours after EDTA.
Lead excretion after DMSA was less than after EDTA, and cumulative excretion after DMSA plateaued at six to eight hours.
The 2 hour and 4 hour cumulative lead excretions after DMSA were highly correlated with the eight hour total.
In multiple linear regression analyses, blood lead was found to be an important predictor of EDTA-chelatable lead, whereas urinary aminolevulinic acid (ALAU) was associated with DMSA-chelatable lead.
Notably, lead excretion after DMSA was greatly increased if EDTA was given first.
Mots-clés Pascal : Plomb, Métal lourd, Exposition professionnelle, Toxicocinétique, Excrétion, Chélateur, EDTA, Etude comparative, Succimer, Surveillance biologique, Urine, Homme, Médecine travail, Méthode étude
Mots-clés Pascal anglais : Lead, Heavy metal, Occupational exposure, Toxicokinetics, Excretion, Chelating agent, EDTA, Comparative study, Biological monitoring, Urine, Human, Occupational medicine, Investigation method
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0118983
Code Inist : 002B03L05. Création : 09/06/1995.