A whole blood assay is described to measure T cell mediated immune responses to leprosy and provide an alternative to the conventional lymphocyte transformation test.
Optimal conditions were defined for the whole blood assay, and interferon-gamma measurement was found to be a more sensitive way of measuring responses than tritiated thymidine incorporation.
The assay was shown to be useful for investigating responses to a range of leprosy antigens.
A whole blood assay has the advantages of being quick, simple and requiring only a small volume of blood, making it more appropriate as an immuno-epidemiological field test in leprosy endemic areas.
Mots-clés Pascal : Lymphocyte T, Interféron gamma, Réponse immune, Antigène, Epidémiologie, Immunogénicité, Sang, Lèpre, Mycobactériose, Bactériose, Infection, Peau pathologie
Mots-clés Pascal anglais : T-Lymphocyte, Gamma interferon, Immune response, Antigen, Epidemiology, Immunogenicity, Blood, Leprosy, Mycobacterial infection, Bacteriosis, Infection, Skin disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0087710
Code Inist : 002B05B02L1. Création : 09/06/1995.