Western blot as a seroepidemiologic tool for detecting foci of Mediterranean spotted fever (MSF).
We conducted a serosurvey on Mediterranean spotted fever (MSF), in a nonendemic area using western blot and microimmunofluorescence.
Among 262 tested sera, 53 were positive by microimmunofluorescence at a titer of 50.
When 48 positive sera were western blot tested, 15 did not exhibited any reaction, 17 reacted against the non-specific lipopolysaccharide, and only 16 reacted against the specific protein antigens.
Fourteen of the sera with a specific reaction were sampled in a village with a unique submediterranean climate.
Western blot may be a more specific tool to determine the real seroprevalence of MSF.
Mots-clés Pascal : Fièvre boutonneuse, Rickettsiose, Rickettsialose, Bactériose, Infection, Epidémiologie, Sérologie, Méthode étude, Immunofluorescence, Méthode immunoblotting, Etude comparative, Homme, Europe, Sud
Mots-clés Pascal anglais : Boutonneuse fever, Rickettsial infection, Rickettsialosis, Bacteriosis, Infection, Epidemiology, Serology, Investigation method, Immunofluorescence, Immunoblotting assay, Comparative study, Human, Europe, South
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 94-0588404
Code Inist : 002B05B02L8. Création : 09/06/1995.