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  1. Evaluation of assays for the identification and quantitation of muconic acid, a benzene metabolite in human urine.

    Article - En anglais

    Muconic acid (MA) is a urinary metabolite of benzene and has been used as a biomarker of exposure to benzene in humans exposed to levels as low as 1 ppm.

    We have modified a high-pressure liquid chromatography (EPIC) based assay for urinary MA (Ducos et al., 1990) by the use of a diode array detector.

    This modification increases the specificity of the EPIC-based assay by identifying false positives.

    In addition, we have developed a gas chromatography (GC) based assay that uses a flame ionization detector (GC-FID).

    Both assays identified and quantified MA in human urine at concentrations greater than 40-50 ng/ml.

    Assay precision was within 10% relative standard deviation for MA concentrations above 90 ng/ml using the EPIC assay and above 40 ng/ml using the GC-FID assay.

    Mots-clés Pascal : Benzène, Métabolite, Exposition professionnelle, Surveillance biologique, Marqueur biologique, Analyse quantitative, Urine, Homme, Liquide biologique, Médecine travail, Chromatographie HPLC, Muconique acide

    Mots-clés Pascal anglais : Benzene, Metabolite, Occupational exposure, Biological monitoring, Biological marker, Quantitative analysis, Urine, Human, Biological fluid, Occupational medicine, HPLC chromatography

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 94-0537846

    Code Inist : 002B03L04. Création : 199501.