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  1. Evaluation of a commercial DNA purification system for plasmid analysis of nosocomial bacterial pathogens.

    Article - En anglais

    This study evaluated the Promega Magic Minipreps (MM) (Promega Corporation, Madison, WI) DNA purification system for use in plasmid analysis of common nosocomial bacterial pathogens.

    The MM system is a kit that includes lysis solutions and buffers and incorporates a minicolumn of DNA binding resin for recovery of plasmid DNA.

    The MM system was used according to the manufacturer's directions to recover plasmids for agarose gel electrophoresis from clinical isolates of Acinetobacter calcoaceticus, Enterobacter cloacae, and Klebsiella pneumoniae.

    For Salmonella enteritidis and Staphylococcus aureus, lysozyme and lysostaphin, respectively, were used for pretreatment.

    Mots-clés Pascal : Infection, Bactériose, Diagnostic, Technique, Homme, Exploration microbiologique, Plasmide, DNA, Purification, Electrophorèse gel, Infection nocosomiale

    Mots-clés Pascal anglais : Infection, Bacteriosis, Diagnosis, Technique, Human, Microbiological investigation, Plasmid, DNA, Purification, Gel electrophoresis

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 94-0499420

    Code Inist : 002A05B14. Création : 199501.