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  1. Determination of hepatitis E virus seroprevalence by using recombinant fusion proteins and synthetic peptides.

    Article - En anglais

    Recombinant antigens from hepatitis E virus (HEV) open-reading frames 2 and 3 were expressed in Escherichia coli as cytidine monophosphate-2-keto-3-deoxyoctulosonic acid synthetase (CKS) fusion proteins, purified, and used to develop an EIA for the detection of antibodies.

    Serologic results were compared with those of previous assays by testing 102 samples from an HEV outbreak in Somalia.

    This CKS/HEV EIA detected anti-HEV in all 97 sera found reactive previously and in an additional 2 samples, which were shown to be true HEV-positive samples by supplemental peptide and Western blot tests.

    The CKS/HEV EIA and supplemented assays were then used to determine seroprevalence of HEV worldwide.

    Mots-clés Pascal : Virus hépatite E, Virus, Cadre lecture ouvert, Technique ELISA, Anticorps, Europe, Prévalence, Protéine recombinante, Peptide, Afrique, Somalie, Epidémiologie, Homme, Sérum, Exploration immunologique, Foie pathologie, Appareil digestif pathologie, Hépatite virale E, protéine fusion

    Mots-clés Pascal anglais : Hepatitis E virus, Virus, Open reading frame, ELISA assay, Antibody, Europe, Prevalence, Recombinant protein, Peptides, Africa, Somali Republic, Epidemiology, Human, Serum, Immunological investigation, Hepatic disease, Digestive diseases

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 94-0473704

    Code Inist : 002B05C02G. Création : 199501.