A standard method for measurement of airborne environmental endotoxin was developed and field tested in a fiberglass insulation-manufacturing facility.
This method involved sampling with a capillary-pore membrane filter, extraction in buffer using a sonication bath, and analysis by the kinetic Limulus assay with resistant-parallel-line estimation (KLARE).
Cross-validation of the extraction and assay method was performed by comparison with methanolysis of samples followed by 3-hydroxy fatty acid (3-OHFA) analysis by gas chromatography-mass spectrometry.
Direct methanolysis of filter samples and methanolysis of buffer extracts of the filters yielded similar 3-OHFA content (P=0.72); the average difference was 2.1%.
Mots-clés Pascal : Endotoxine, Méthode, Analyse quantitative, Aérosol, Méthode cinétique, Essai biologique, Détection, Limulus, Acinetobacter johnsonii, Deleya aesta, Merostomata, Arthropoda, Invertebrata, Neisseriaceae, Micrococcales, Bactérie, Halomonadaceae
Mots-clés Pascal anglais : Endotoxin, Method, Quantitative analysis, Aerosols, Kinetic method, Bioassay, Detection, Limulus, Merostomata, Arthropoda, Invertebrata, Neisseriaceae, Micrococcales, Bacteria, Halomonadaceae
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 94-0341507
Code Inist : 002A05B14. Création : 199406.