Monitoring of bacterial sugars and hydroxy fatty acids in dust from air conditioners by gas chromatography-mass spectrometry.
Bacterial levels in dust collected from hospital air-conditioning filters were examined by chemical analysis (without prior culture).
The dust was analyzed by gas chromatography-mass spectrometry after hydrolysis and derivatization.
L-Glycero-D-mannoheptose and hydroxy fatty acids (3-OH 12:0 and 3-OH 14:0) (primarily found in lipopolysaccharide) and muramic acid (a chemical marker for bacterial peptidoglycan) were present at higher levels in dust collected from filters primarily contacting outdoor (as opposed to indoor) air.
The ratio of L-glycero-D-mannoheptose to muramic acid in dust (compared with those of a group of gram-positive and gram-negative bacteria) suggested that both dust types confined appreciable numbers of gram-negative bacteria.
Mots-clés Pascal : Chromatographie phase gazeuse, Spectrométrie masse, Conditionneur air, Poussière, Acide gras, Glucide, Filtre air, Bactérie Gram négatif, Analyse quantitative, Origine microbienne, Bactérie Gram positif, Milieu hospitalier, Qualité air
Mots-clés Pascal anglais : Gas chromatography, Mass spectrometry, Air conditioner, Dust, Fatty acids, Carbohydrate, Air filter, Gram negative bacteria, Quantitative analysis, Microbial origin, Gram positive bacteria, Hospital environment, Air quality
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 94-0190884
Code Inist : 002B30A02C. Création : 199406.