Direct detection of wild poliovirus circulation by stool surveys of healthy children and analysis of community wastewater.
Cartagena, Colombia, was one of the last cities in the Americas known to have endemic poliomyelitis.
After 3 cases were identified in 1991, two approaches for detecting continued silent transmission of wild polioviruses within a high-risk community were used: stool surveys of healthy children and virologic analysis of community sewage.
Wild type I polioviruses were isolated from 8% of the children studied and from 21 ; of sewage samples.
The proportions of wild polioviruses, vaccine-related polioviruses, and nonpolio enteric viruses were similar for both approaches.
Wild poliovirus sequences were also amplified directly from processed sewage samples by the polymerase chain reaction using primer pairs specific for the indigenous type 1 genotype.
Mots-clés Pascal : Poliovirus, Enterovirus, Picornaviridae, Virus, Surveillance sanitaire, Dépistage, Exploration microbiologique, Epidémiologie, Colombie, Amérique du Sud, Amérique, Biologie moléculaire, Transmission, Fèces, Enfant, Homme, Réaction chaîne polymérase, Génotype
Mots-clés Pascal anglais : Poliovirus, Enterovirus, Picornaviridae, Virus, Sanitary surveillance, Medical screening, Microbiological investigation, Epidemiology, Colombia, South America, America, Molecular biology, Transmission, Feces, Child, Human, Polymerase chain reaction, Genotype
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 94-0134438
Code Inist : 002B05C02A. Création : 199406.