Molecular typing of Enterobacter cloacae by pulsed-field gel electrophoresis of genomic restriction fragments.
Eleven multiply resistant Enterobacter cloacae isolates were obtained from eight preterm neonates in a neonatal intensive care unit (NICU) of one hospital in Osnabrück, together with one sensitive strain from another infant.
The presence of similar antibiograms and biotypes in 11 isolates prompted further characterization of the isolates by pulsed-field gel electrophoresis (PFGE) of NotI generated genomic restriction fragments.
For assessment of the discriminatory power of this typing method 50 non-related strains were included in the study.
Non-related strains demonstrated a marked variation in restriction fragment patterns and were clearly discriminated one from another.
Mots-clés Pascal : Enterobacter cloacae, Enterobacteriaceae, Bactérie, Epidémiologie, Electrophorèse champ pulsé, Prématuré, Nouveau né, Homme, Electrophorèse gel, Unité soin intensif, Hôpital, Test sensibilité médicamenteuse, Typage, Fragment restriction, infection nosocomiale
Mots-clés Pascal anglais : Enterobacter cloacae, Enterobacteriaceae, Bacteria, Epidemiology, Pulsed field electrophoresis, Premature, Newborn, Human, Gel electrophoresis, Intensive care unit, Hospital, Drug susceptibility test, Typing, Restriction fragment
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 94-0065548
Code Inist : 002B27B14C. Création : 199406.