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  1. Analysis of acquired human cytomegalovirus infection by polymerase chain reaction.

    Article - En anglais

    We used the polymerase chain reaction and primers corresponding to three regions of the human cytomegalovirus (HCMV) genome to study HCMVs isolated from 16 children attending a single day-care center and the father of two children in the same center.

    When we analyzed isolates with primers for the pp65 and major immediate-early genes, we observed nearly uniform amplification yielding products of predicted sizes.

    By contrast, primers for the a sequence demonstrated variability among HCMV strains, supporting the use of these primers as an epidemiologic tool.

    Analysis of a-sequence products from two isolates demonstrated 50 to 70% nucleotide homology with the a sequence of HCMV Towne strain DNA.

    Mots-clés Pascal : Enfant, Cytomegalovirus humain, Méthode, Détection, Séquence nucléotide, Etude familiale, Etude comparative, Alignement séquence, Homologie, Garderie enfant, Epidémiologie, Homme, Betaherpesvirinae, Herpesviridae, Virus

    Mots-clés Pascal anglais : Child, Human cytomegalovirus, Method, Detection, Nucleotide sequence, Family study, Comparative study, Sequence alignment, Homology, Day care center, Epidemiology, Human, Betaherpesvirinae, Herpesviridae, Virus

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 93-0673420

    Code Inist : 002B30A01A2. Création : 199406.