Comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for Torulopsis glabrata and Candida species other than C. albicans.
Candida species have recently emerged as important nosocomial pathogens.
Because of the lack of a reliable system for detecting differences within the same species, little is known about the epidemiology of infection with Candida species.
We describe a typing system for Torulopsis glabrata and the non-C. albicans Candida species that uses contour-clamped homogeneous electric field electrophoresis (CHEF), a version of pulsed-field gradient gel electrophoresis, and compared it with restriction enzyme analysis (REA) of genomic DNA.
One hundred seventeen clinical isolates from 40 patients were evaluated.
CHEF and REA were performed on each of the isolates, and the results of the two procedures were compared.
Mots-clés Pascal : Candida albicans, Fungi Imperfecti, Fungi, Thallophyta, Levure, Isolat clinique, Homme, Typage, Epidémiologie, Etude comparative, Candidose, Mycose, Infection, Electrophorèse champ pulsé, Carte restriction, Hôpital, Torulopsis, Torulopsis glabrata
Mots-clés Pascal anglais : Candida albicans, Fungi Imperfecti, Fungi, Thallophyta, Yeast, Clinical isolate, Human, Typing, Epidemiology, Comparative study, Candidiasis, Mycosis, Infection, Pulsed field electrophoresis, Restriction map, Hospital, Torulopsis
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0653215
Code Inist : 002B05D02L. Création : 199406.