Detection of hepatitis E virus in raw and treated wastewater with the polymerase chain reaction.
The main objective of this study was to determine the applicability of the polymerase chain reaction (PCR) to detection of hepatitis E virus (HEV) in sewage treatment plants and establishment of the prevalence of hepatitis viral diseases in a population.
Epidemics of HEV infection because of inadequate public sanitation have been reported in several developing countries.
A procedure for concentration of HEV in sewage samples through adsorption to membrane filters, elution with urea-arginine phosphate buffer, and subsequent reconcentration with magnesium chloride enabled us to concentrate HEV to volumes in the microliter range.
HEV-specific cDNA was prepared by reverse transcription of the total RNA extracted from samples.
Mots-clés Pascal : Détection, Eau usée, Station épuration, Concentration, Filtre membrane, Réaction chaîne polymérase, Amplification, DNA complémentaire, Virus, Virus hépatite E
Mots-clés Pascal anglais : Detection, Waste water, Sewage treatment plant, Concentration, Membrane filter, Polymerase chain reaction, Amplification, Complementary DNA, Virus, Hepatitis E virus
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0653188
Code Inist : 002A05C09. Création : 199406.