The main objective of this study was to determine the applicability of the polymerase chain reaction (PCR) to detection of hepatitis E virus (HEV) in sewage treatment plants and establishment of the prevalence of hepatitis viral diseases in a population.
Epidemics of HEV infection because of inadequate public sanitation have been reported in several developing countries.
A procedure for concentration of HEV in sewage samples through adsorption to membrane filters, elution with urea-arginine phosphate buffer, and subsequent reconcentration with magnesium chloride enabled us to concentrate HEV to volumes in the microliter range.
HEV-specific cDNA was prepared by reverse transcription of the total RNA extracted from samples.
Mots-clés Pascal : Détection, Eau usée, Station épuration, Concentration, Filtre membrane, Réaction chaîne polymérase, Amplification, DNA complémentaire, Virus, Virus hépatite E
Mots-clés Pascal anglais : Detection, Waste water, Sewage treatment plant, Concentration, Membrane filter, Polymerase chain reaction, Amplification, Complementary DNA, Virus, Hepatitis E virus
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0653188
Code Inist : 002A05C09. Création : 199406.