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  1. Detection of hepatitis E virus in raw and treated wastewater with the polymerase chain reaction.

    Article - En anglais

    The main objective of this study was to determine the applicability of the polymerase chain reaction (PCR) to detection of hepatitis E virus (HEV) in sewage treatment plants and establishment of the prevalence of hepatitis viral diseases in a population.

    Epidemics of HEV infection because of inadequate public sanitation have been reported in several developing countries.

    A procedure for concentration of HEV in sewage samples through adsorption to membrane filters, elution with urea-arginine phosphate buffer, and subsequent reconcentration with magnesium chloride enabled us to concentrate HEV to volumes in the microliter range.

    HEV-specific cDNA was prepared by reverse transcription of the total RNA extracted from samples.

    Mots-clés Pascal : Détection, Eau usée, Station épuration, Concentration, Filtre membrane, Réaction chaîne polymérase, Amplification, DNA complémentaire, Virus, Virus hépatite E

    Mots-clés Pascal anglais : Detection, Waste water, Sewage treatment plant, Concentration, Membrane filter, Polymerase chain reaction, Amplification, Complementary DNA, Virus, Hepatitis E virus

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 93-0653188

    Code Inist : 002A05C09. Création : 199406.