Estimation of local vital reactions in severely burned tissues of guinea pig skin using C3a or C3a desArg as a marker.
We observed changes in levels of anaphylatoxin C3a and/or its desArg (C3a/C3a desArg) peptides in the local site of severely burned skin tissues of guinea pigs using immuno-Western blotting methods.
The C3a/C3a desArg peptides, which were probably generated during complement activation, were detected at significant levels from 30 min to 72 h following burn injury in an area limited to 5 mm from the wound edge.
Levels of these peptides showed a tendency to be highest in that area 24 h after burn infliction.
In postmortem injuries, C3a/C3a desArg peptides could not be detected.
These peptides were detected in antemortem wounds up to at least 2 days at 22oC and up to 5 days at 4oC after death, although decreases in levels were found.
Mots-clés Pascal : Cobaye, Rodentia, Mammalia, Vertebrata, Peau, Anaphylatoxine, Animal, Brûlure, Méthode immunoblotting, Peptide, Marqueur biologique, Inflammation, Aspect médicolégal, Complèment C3a
Mots-clés Pascal anglais : Guinea pig, Rodentia, Mammalia, Vertebrata, Skin, Anaphylatoxin, Animal, Burn, Immunoblotting assay, Peptides, Biological marker, Inflammation, Forensic aspect, 3a Complement
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0626303
Code Inist : 002B30A10. Création : 199406.