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  1. Purification of blood-group substances in human hepatic bile (HHB) and immunological characterization of anti-HHB serum.

    Article - En anglais

    Blood-group substances (BGS) in human hepatic bile (HHB) of blood type A were purified 3657-7314-fold by ethanol sedimentation and column chromatography with DEAE Sephadex A-50 and Concanavalin A Sepharose 4B.

    An anti-HHB serum was raised by immunizing rabbits with the purified BGS in the HHB.

    Cross-reactions of the native anti-HHB serum with such human materials as type A blood cell, plasma, seminal fluid and saliva were eliminated by stepwise sequential absorptions with type A red blood cells, type 0 seminal fluid and type AB plasma.

    Precipitin titers of the native anti-HHB serum were x320 and x80 and those of the absorbed anti-HHB serum x80 and x20 against the immunogen and HHB, respectively.

    Mots-clés Pascal : Substance groupe sanguin, Foie, Feces, Purification, Identification, Bile, Homme, Méthode immunologique, Spécificité espèce, Médecine légale, Système ABO(H)

    Mots-clés Pascal anglais : Blood group substance, Liver, Feces, Purification, Identification, Bile, Human, Immunological method, Species specificity, Legal medicine, ABO(H)-System

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 93-0626300

    Code Inist : 002B30A10. Création : 199406.