Purification of blood-group substances in human hepatic bile (HHB) and immunological characterization of anti-HHB serum.
Blood-group substances (BGS) in human hepatic bile (HHB) of blood type A were purified 3657-7314-fold by ethanol sedimentation and column chromatography with DEAE Sephadex A-50 and Concanavalin A Sepharose 4B.
An anti-HHB serum was raised by immunizing rabbits with the purified BGS in the HHB.
Cross-reactions of the native anti-HHB serum with such human materials as type A blood cell, plasma, seminal fluid and saliva were eliminated by stepwise sequential absorptions with type A red blood cells, type 0 seminal fluid and type AB plasma.
Precipitin titers of the native anti-HHB serum were x320 and x80 and those of the absorbed anti-HHB serum x80 and x20 against the immunogen and HHB, respectively.
Mots-clés Pascal : Substance groupe sanguin, Foie, Feces, Purification, Identification, Bile, Homme, Méthode immunologique, Spécificité espèce, Médecine légale, Système ABO(H)
Mots-clés Pascal anglais : Blood group substance, Liver, Feces, Purification, Identification, Bile, Human, Immunological method, Species specificity, Legal medicine, ABO(H)-System
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0626300
Code Inist : 002B30A10. Création : 199406.