Comparison of Western blot and microimmunofluorescence as tools for lyme disease seroepidemiology.
Sera from a population of 212 farmers of the Puy de Dome (Midlands of France) who are in close contact with Ixodes ricinus, the vector of Lyme disease in Europe, and sera from 100 urban blood donors from the nearly city of Clermont Ferrand were examined by microimmunofluorescence (MIF) for antibodies reacting with Borrelia bugdorferi.
The results showed a higher seroprevalence of IgG>1/100 in farmers (25oC) than in blood donors (10%). Using western blot with antibody at a 1/200 dilution, and regarding sera as being positive if they contained IgG reactive with more than 5 bands, 93/212 farmers (44%) and 20% of blood donors were positive.
Reactions with specific protein bands (94, 73, 30 and 21 KDa) by western blot were demonstrated in 83/212 sera from farmers (39%) and 16% of blood donors.
Mots-clés Pascal : Lyme maladie, Borréliose, Spirochétose, Bactériose, Infection, Borrelia burgdorferi, Spirochaetaceae, Spirochaetales, Bactérie, Epidémiologie, Sérologie, Homme, Prévalence, Agriculture, Milieu urbain, Méthode mesure, Méthode immunoblotting, Immunofluorescence, Etude comparative, France, Europe
Mots-clés Pascal anglais : Lyme disease, Borrelia infection, Spirachaetosis, Bacteriosis, Infection, Borrelia burgdorferi, Spirochaetaceae, Spirochaetales, Bacteria, Epidemiology, Serology, Human, Prevalence, Agriculture, Urban environment, Measurement method, Immunoblotting assay, Immunofluorescence, Comparative study, France, Europe
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0540019
Code Inist : 002B05B02L6. Création : 199406.