A protozoological survey was done to isolate free-living amoebae from swimming pools and physiotherapy tubs in Mexico City.
Amoebae were isolated by culture in nonnutritive agar medium combined with Escherichia coli.
Identification of the isolates was done by morphology using specialized taxonomic keys and by isoelectric focusing of total proteins and isoenzymes in agarose.
Pathogenicity of the isolates was determined in 3-week-old mice by intracerebral and intranasal inoculations.
Statistical tools were used to determine the existence of significant differences on the incidence and diversity of the isolates in relation to the two types of water bodies analyzed.
Water and environmental temperatures, pH, and chloride concentrations were determined at each sampling site.
Mots-clés Pascal : Mexique, Amérique Centrale, Amérique, Qualité eau, Piscine, Isolement, Naegleria, Rhizoflagellata, Protozoa, Acanthamoeba, Hygiène, Identification
Mots-clés Pascal anglais : Mexico, Central America, America, Water quality, Swimming pool, Isolation, Naegleria, Rhizoflagellata, Protozoa, Acanthamoeba, Hygiene, Identification
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0529887
Code Inist : 002B30A02C. Création : 199406.