Employees of a biotechnological production plant for recombinant interferonalpha-2a have been participating in a 1-year study on occupational hygiene and the resulting biosatefy aspects.
Most of the employees have been employed in the plant for more than 6 years.
Weekly stool samples were analysed for tetracycline (used as selection marker)-resistant coliforms as well as for rDNA (IFN gene) (interferon gene) and for the production organism.
Various analytical methods, including the polymerase chain reaction, were applied to show that neither rDNA nor the production organism could be found in any of the stool samples and that there was no change or trend in the gut flora with respect to tetracycline resistance.
Mots-clés Pascal : Exposition professionnelle, Sang, Surveillance biologique, Réaction chaîne polymérase, Hygiène travail, Industrie pharmaceutique, Production, Biotechnologie, Interféron alpha, DNA recombinant, Microflore, Intestin, Résistance, Tétracycline, Anticorps, Cytokine
Mots-clés Pascal anglais : Occupational exposure, Blood, Biological monitoring, Polymerase chain reaction, Occupational hygiene, Pharmaceutical industry, Production, Biotechnology, Alpha interferon, Recombinant DNA, Microflora, Gut, Resistance, Antibody, Cytokine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0443758
Code Inist : 002B30B01. Création : 199406.