This study of the effect on CD4%, CD8%, CD3+ 8+, and CD3% of flow cytometer, monoclonal antibody, and fluorochrome was based on 71 whole-blood samples, each evaluated by 42 to 59 laboratories during 2 years of a flow cytometry quality assessment program.
For the 24 HIV-positive specimens, FACScans produced significantly lower CD4% values than EPICS-Cs or EPICS Profiles, and for the 47 HIV-negative specimens, FITC was associated with significantly lower CD4% values than PE or RD1, but differences were never larger than 2% and regressions accounted for only 3-12% of the variability.
Mots-clés Pascal : Homme, Méthode, Appareillage, Anticorps monoclonal, Cytométrie flux, Détection, Immunodéficit acquis syndrome, Virose, Infection, Immunopathologie, Hémopathie, Phénotype, Etude comparative, Evaluation performance, Lymphocyte T, Sous population cellulaire, Antigène CD8, Antigène CD3, Antigène CD4
Mots-clés Pascal anglais : Human, Method, Instrumentation, Monoclonal antibody, Flow cytometry, Detection, AIDS, Viral disease, Infection, Immunopathology, Hemopathy, Phenotype, Comparative study, Performance evaluation, T-Lymphocyte, Cell subpopulation
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0431936
Code Inist : 002B06D01. Création : 199406.