Somatic p53 mutations in human breast carcinomas in an Icelandic population : a prognostic factor.
Mutations in the p53 gene are among the most common genetic changes in human carcinomas.
They have been found in many tumor types including colon, lung, and breast.
We have used constant denaturant gel electrophoresis in order to screen samples from 109 breast carcinomas for mutations in four conserved regions, exons 5, 7, and 8, of the p53 gene.
Samples were also analyzed for allelic loss of the p53 gene and of markers more distal on chromosome 17p.
Mutations were confirmed by DNA sequencing.
Mutations were found in 18 of the 109 samples (16.5%). Loss of heterozygosity at 17p was detected in the majority of informative mutated cases.
All cases were also screened for germ line mutations, but none were found.
Mots-clés Pascal : Epithélioma, Tumeur maligne, Glande mammaire, Glande mammaire pathologie, Homme, Exploration, Mutation, Pathogénie, Pronostic, Biologie moléculaire, Islande, Iles Atlantiques, Epidémiologie, Chromosome E17 anormal, Chromosome anormal, Aberration chromosomique, Gène p53, Perte hétérozygotie, Gène suppresseur tumeur
Mots-clés Pascal anglais : Carcinoma, Malignant tumor, Mammary gland, Mammary gland diseases, Human, Exploration, Mutation, Pathogenesis, Prognosis, Molecular biology, Iceland, Atlantic Ocean Islands, Epidemiology, Abnormal E17 chromosome, Abnormal chromosome, Chromosomal aberration, Tumor suppressor gene
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0409460
Code Inist : 002B20E02. Création : 199406.