In Germany, transmission of Hepatitis C virus by blood transfusion is prevented by screening the donations for anti-HCV and ALT.
The specificity of the anti-HCV screening in low seroprevalence populations has been questioned.
In order to evaluate this screening policy we wanted to estimate the prevalence of viremic and potentially infectious donors by the HCV-RNA polymerase chain reaction (PCR) in our donor population of southern Germany.
Donors (n=301) were divided into four subgroups according to anti-HCV status and ALT levels.
HCV sequences were detected by nested PCR, using primers for the most conserved region of the viral genome.
The recombinant immunoblot assay (RIBA-4) was applied to the same samples.
Mots-clés Pascal : Hépatite virale C, Virose, Infection, Virus hépatite C, Virus, Diagnostic, Prévalence, RNA, Appareil digestif pathologie, Foie pathologie, Donneur sang, Allemagne, Europe, Etude comparative, Epidémiologie, Réaction chaîne polymérase, DNA polymerase RNA-dependent, Enzyme, Technique ELISA, Méthode immunoblotting, Biologie moléculaire, Exploration, Méthode immunoenzymatique, Homme
Mots-clés Pascal anglais : Viral hepatitis C, Viral disease, Infection, Hepatitis C virus, Virus, Diagnosis, Prevalence, RNA, Digestive diseases, Hepatic disease, Blood donor, Germany, Europe, Comparative study, Epidemiology, Polymerase chain reaction, DNA polymerase RNA-dependent, Enzyme, ELISA assay, Immunoblotting assay, Molecular biology, Exploration, Enzyme immunoassay, Human
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0393192
Code Inist : 002B05C02F. Création : 199406.