Detection of human proteins in buried blood using Elisa and monoclonal antibodies : towards the reliable species indentification of blood stains on buried material.
The survival of human proteins in blood stains on fragments of cloth buried in exposed soil was examined in a 15-month investigation carried out from September 1990 to December 1991.
During this period there was a wide variety of weather conditions.
Samples were exhumed at 4-weekly intervals for 16 weeks and finally at 65 weeks; extracts of the stains were tested for albumin and IgG using a highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) performed with monoclonal antibodies.
Human albumin survived well throughout the 15 months of study, but IgG could be detected only in the 4- and 8-week samples.
Mots-clés Pascal : Tache, Protéine sérique, Stabilité, Médecine légale, Sang, Vêtement, Marqueur biologique, Détection, Long terme, Sol, Exhumé, Homme, Technique ELISA, Albumine, IgG
Mots-clés Pascal anglais : Stain, Serum protein, Stability, Legal medicine, Blood, Clothing, Biological marker, Detection, Long term, Soils, Human, ELISA assay, Albumin, IgG
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 93-0201283
Code Inist : 002B30A10. Création : 199406.