DNA-adduct formation, depurination and imidazole ringopening were followed in vitro using styrene oxide, ethyleneimine and dimethyl sulfate.
Depurination was found to be about 50 times faster in nucleosides than in double-stranded DNA.
This apparent discrepancy in depurination between alkylated and intact bases suggests the possibility of developing a sensitive method for monitoring of DNA alkylations formed by electrophillic chemicals, which might be based on labelling of apurinic sites and utilized for in vivo studies as well.
Mots-clés Pascal : Exposition professionnelle, Hygiène, Carcinogène, Dépurination, Alkylation, DNA, Xénobiotique, Contrôle, Méthodologie
Mots-clés Pascal anglais : Occupational exposure, Hygiene, Carcinogen, Depurination, Alkylation, DNA, Xenobiotic, Check, Methodology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 91-0237563
Code Inist : 001D16C06. Création : 199406.