The present study was initiated in order to identify the best marker of occupational exposure to cyclohexanone among cyclohexanone and its metabolites in urine.
To examine if diffusive samplers are applicable to personal monitoring of exposure to cyclohexanone in workroom air, the performance of carbon cloth to adsorb cyclohexanone in air was studied by experimental exposure of the cloth to cyclohexanone at 5,10,25 or 50 ppm (i.e. 20,40,100 or 200 mg m-3) for up to 8 h. Cyclohexanone in the exposed cloth was extracted with carbon disulphide followed by gas chromatographic (GC) analysis.
The cloth adsorbed cyclohexanone in proportion to the concentration (up to 50 ppm) and the duration (up to 8 h), and responded quantitatively to a 15 min exposure at 100 ppm.
In a field survey, end-of-shift urine samples were collected from 24 factory workers occupationally exposed to cyclohexanone (up to 9 ppm) in combination with toluene and other solvents.
Urine samples were also collected from 10 subjects with no occupational exposure to solvents.
The urine samples were treated with acid or an enzyme preparation for hydrolysis, and extracted with dichloromethane or ethyl acetate.
The extracts were analysed by GC for cyclohexanone, cyclohexanol, and trans-and cis-isomers of 1,2-and 1,4-cyclohexanediol.
Both cyclohexanol and trans-1,2-cyclohexanediol in urine correlated significantly with time-weighted average intensity of exposure to cyclohexanone. (...)
Mots-clés Pascal : Cyclohexanone, Lieu travail, Solvant organique, Exposition professionnelle, Médecine travail, Homme, Surveillance biologique, Métabolite, Urine, Liquide biologique, Mesure, Analyse quantitative, Analyse chimique, Teneur air ambiant, Processus diffusion, Prélèvement, Piégeage, Chromatographie phase gazeuse, Marqueur biologique, Echantillonnage, Charbon
Mots-clés Pascal anglais : Cyclohexanone, Work place, Organic solvent, Occupational exposure, Occupational medicine, Human, Biological monitoring, Metabolite, Urine, Biological fluid, Measurement, Quantitative analysis, Chemical analysis, Ambient air concentration, Diffusion process, Samplings, Trapping, Gas chromatography, Biological marker, Sampling, Coal
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0519513
Code Inist : 002B03L04. Création : 18/05/2000.