A total of 202 serum and stool samples from acute hepatitis patients attending the Fever Hospital of Alexandria, Egypt, have been studied to reveal markers of hepatitis virus infection.
Anti-HAV IgM were detected in 21 out of 202 sera (10.4%), whereas 201 sera (99.5%) had anti-HAV IgG.
The first age attack was in the class-age 0-9 years with 64.7% of anti-HAV IgM positive sera.
Among 202 patients, anti-hepatitis E IgG (sample/over cut off>1.0) was identified in 90 patients (44.5%). The anti-HEV seropositivity ranged from 17.6% to 60.0% in the different age groups, with the highest level in the class-age 20-29 years.
Anti-hepatitis E IgM were identified in 49 patients with the first age attack in the class-age 10-19 years (39.4%). HAV RNA was identified by nested PCR in 7 samples out of 15, whereas HEV RNA was present in 4 out of 75 stool samples.
Direct DNA sequence of the latter PCR products confirmed the presence of the HEV genome ; comparison of the sequences of the isolates from Egypt with those in data banks revealed the highest homology to the Burma strain.
Our data confirm that HAV and HEV are common causes of acute sporadic hepatitis in Alexandria but with different peak age positivity.
Occasionally, but not infrequently, dual infections (HAV-HEV and HEV-enteric viruses) were also found. (...)
Mots-clés Pascal : Hépatite virale A, Virose, Infection, Hépatite virale E, Hospitalisation, IgG, IgM, Plasma sanguin, Sérologie, Anticorps, Réaction chaîne polymérase, Biologie moléculaire, Epidémiologie, Prévalence, Diagnostic, Homme, Egypte, Afrique, Appareil digestif pathologie, Foie pathologie
Mots-clés Pascal anglais : Viral hepatitis A, Viral disease, Infection, Viral hepatitis E, Hospitalization, IgG, IgM, Blood plasma, Serology, Antibody, Polymerase chain reaction, Molecular biology, Epidemiology, Prevalence, Diagnosis, Human, Egypt, Africa, Digestive diseases, Hepatic disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0515785
Code Inist : 002B05C02G. Création : 18/05/2000.