Permeability tests with Franz'diffusion cells and an in vitro test model were made to evaluate the importance of dermal absorption of nicotine as a pathway for intoxication.
Studies were carried out to ensure that safety procedures, when spilling nicotine on skin, are sufficient to prevent poisoning.
Pure nicotine and nicotine in various concentrations in water or ethanol were applied on human skin or gloves in Franz'cells.
Washing was simulated by removing nicotine from skin after 3 or 5 min.
Permeation rate (flux) and lag time were calculated and estimated for human skin.
Different glove materials were tested for their nicotine breakthrough time.
Flux depended on concentration in a non-linear way when nicotine-water solutions were tested.
Highest flux was found in 50% w/w nicotine dissolved in water.
Solutions with low concentration of nicotine (1% w/w) dissolved in water had a similar permeation rate to 100% nicotine.
Flux was found to be low when using ethanol as a vehicle ; flux was also pH-dependent.
The nicotine-water solution containing acetic acid had the lowest flux.
The tests where nicotine was washed away revealed that skin served as a possible nicotine depot, because nicotine concentration in the receptor compartment continued to increase after removing the nicotine from the surface. (...)
Mots-clés Pascal : Nicotine, Exposition professionnelle, Médecine travail, Pénétration, Peau, Voie externe, Voie percutanée, Perméabilité, In vitro, Homme, Toxicité, Prévention, Gant, Vêtement protection, Rinçage, Alcaloïde, Insecticide, Pesticide
Mots-clés Pascal anglais : Nicotine, Occupational exposure, Occupational medicine, Penetration, Skin, Topical administration, Percutaneous route, Permeability, In vitro, Human, Toxicity, Prevention, Glove, Safety clothing, Rinsing, Alkaloid, Insecticide, Pesticides
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0513525
Code Inist : 002B03E. Création : 18/05/2000.