In spring and autumn of 1994 duplicates of 24-h diets were collected from 123 respondents.
One of the goals of this study was to determine the amount of nitrite and nitrate in the duplicates of 24-h diets to establish the oral daily intake of these analytes.
For this purpose an HPIC/UV method for the determination of nitrate and nitrite in duplicate diets was developed and validated.
The sample preparation procedure was derived from the in-house method used for the determination of nitrate and nitrite in human blood plasma.
The sample is diluted with water, deproteinized with Carrez reagent, followed by chromatographic clean-up on an SPE C18-column.
Both the nitrate and the nitrite results are quantitative.
The recovery for nitrite was on average 104% (n=21, spiking levels : 0.84-95 mg/kg) and for nitrate on average 103% (N=21, spiking levels : 1.8-404 mg/kg).
Samples of duplicates of 24-h diets were analysed according to the method developed.
The median intake of nitrite calculated from the samples collected in spring 1994 was 0.6mg/person/day (range<0.1-6. 1 mg/person/day).
For the samples collected in autumn 1994 these figures were<0.2 mg/person/day (range<0.1-16mg/person/day).
The mean intake of nitrate was 73mg/person/day (range 7-322mg/person/day) in spring 1994 and 87mg/person/day (range 1-310 mg/person/day) in autumn 1994.
The overall mean intake of nitrate in 1994 was 80mg/person/day. (...)
Mots-clés Pascal : Pays Bas, Europe, Homme, Aliment, Boisson, Etude expérimentale, Régime alimentaire, Doubles, Nitrate, Nitrite, Dose journalière, Chromatographie ionique, Méthode analytique, Nutrition, Echantillonnage, Polluant
Mots-clés Pascal anglais : Netherlands, Europe, Human, Food, Beverage, Experimental study, Diet, Duplicate, Nitrates, Nitrites, Daily dose, Ion chromatography, Analytical method, Nutrition, Sampling, Pollutant
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0512610
Code Inist : 002B30A02A. Création : 18/05/2000.