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  1. The oral nitrate and nitrite intake in The Netherlands : evaluation of the results obtained by HPIC analysis of duplicate 24-hour diet samples collected in 1994.

    Article - En anglais

    In spring and autumn of 1994 duplicates of 24-h diets were collected from 123 respondents.

    One of the goals of this study was to determine the amount of nitrite and nitrate in the duplicates of 24-h diets to establish the oral daily intake of these analytes.

    For this purpose an HPIC/UV method for the determination of nitrate and nitrite in duplicate diets was developed and validated.

    The sample preparation procedure was derived from the in-house method used for the determination of nitrate and nitrite in human blood plasma.

    The sample is diluted with water, deproteinized with Carrez reagent, followed by chromatographic clean-up on an SPE C18-column.

    Both the nitrate and the nitrite results are quantitative.

    The recovery for nitrite was on average 104% (n=21, spiking levels : 0.84-95 mg/kg) and for nitrate on average 103% (N=21, spiking levels : 1.8-404 mg/kg).

    Samples of duplicates of 24-h diets were analysed according to the method developed.

    The median intake of nitrite calculated from the samples collected in spring 1994 was 0.6mg/person/day (range<0.1-6. 1 mg/person/day).

    For the samples collected in autumn 1994 these figures were<0.2 mg/person/day (range<0.1-16mg/person/day).

    The mean intake of nitrate was 73mg/person/day (range 7-322mg/person/day) in spring 1994 and 87mg/person/day (range 1-310 mg/person/day) in autumn 1994.

    The overall mean intake of nitrate in 1994 was 80mg/person/day. (...)

    Mots-clés Pascal : Pays Bas, Europe, Homme, Aliment, Boisson, Etude expérimentale, Régime alimentaire, Doubles, Nitrate, Nitrite, Dose journalière, Chromatographie ionique, Méthode analytique, Nutrition, Echantillonnage, Polluant

    Mots-clés Pascal anglais : Netherlands, Europe, Human, Food, Beverage, Experimental study, Diet, Duplicate, Nitrates, Nitrites, Daily dose, Ion chromatography, Analytical method, Nutrition, Sampling, Pollutant

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0512610

    Code Inist : 002B30A02A. Création : 18/05/2000.