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  1. Detection of methicillin-resistant Staphylococcus aureus (MRSA) from growth on mannitol salt oxacillin agar using PCR for nosocomial surveillance.

    Article - En anglais

    This study evaluated a polymerase chain reaction (PCR) method for detection of methicillin-resistant Staphylococcus aureus (MRSA) in specimens referred for nosocomial surveillance.

    PCR was used to detect the mecA and nuc gene targets using yellow growth on mannitol salt agar containing 6 mg/liter oxacillin (MSO-6) as a source of DNA (N=645).

    The diagnostic values for PCR compared with culture methods were 97% specificity, 100% sensitivity, 96% positive predictive value, and 100% negative predictive value.

    Total cost for PCR per test is $3.62 compared to $4. 77 for culture.

    However, the total cost per specimen is significantly lower due to only 20% of all surveillance specimens producing yellow colonies on MSO-6.

    The average turnaround time for the PCR method is 48 h compared with 82 h for culture.

    PCR amplification of mecA and nuc genes using yellow colonies on MSO-6 is a simple, fast, accurate and cost-effective method for routine use in clinical laboratories for detecting MRSA in surveillance specimens.

    Mots-clés Pascal : Staphylococcus aureus, Micrococcaceae, Micrococcales, Bactérie, Homme, Staphylococcie, Bactériose, Infection, Infection nosocomiale, Surveillance sanitaire, Méthode, Diagnostic, Détection, Sensibilité résistance, Antibactérien, Antibiotique, Réaction chaîne polymérase, Méticilline, Nuclease, Esterases, Hydrolases, Enzyme, Pénicilline dérivé, bêta-Lactamines, Souche résistante méticilline, Gène mecA, Gène nuc

    Mots-clés Pascal anglais : Staphylococcus aureus, Micrococcaceae, Micrococcales, Bacteria, Human, Staphylococcal infection, Bacteriosis, Infection, Nosocomial infection, Sanitary surveillance, Method, Diagnosis, Detection, Sensitivity resistance, Antibacterial agent, Antibiotic, Polymerase chain reaction, Meticillin, Nuclease, Esterases, Hydrolases, Enzyme, Penicillin derivatives, bêta-Lactams

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0505796

    Code Inist : 002B30A01C. Création : 22/03/2000.