A high prevalence of TT virus (TTV), a novel virus recently identified in the serum of a patient with post-transfusion hepatitis of unknown aetiology, has been reported in blood donors worldwide.
We investigated the presence of TTV DNA in several lots of blood products and in the corresponding plasma pools.
In the process, we determined, from three sets of primers, the one which was most efficient in detecting the viral nucleic acid.
This set amplifies the region closest to the 3'-end of the TTV genome which was proved, by sequence analysis, to be more conserved than the other two regions.
Whereas all 10 intravenous immunoglobulin and 21 albumin batches were TTV negative, 4/5 factor VIII concentrates and 4/10 intramuscular immunoglobulin batches were TTV positive.
A high prevalence of TTV DNA (70%) was found in the plasma pools that were collected from four different countries.
These results confirm the worldwide distribution of this virus and show that TTV is removed with a varying efficiency during the manufacture of blood products.
Mots-clés Pascal : Hépatite virale, Virose, Infection, Plasma sanguin, Sang, Constituant sang, Prévalence, Etude statistique, Biologie moléculaire, Détection, Transmission homme homme, Facteur risque, Homme, Italie, Europe, Appareil digestif pathologie, Foie pathologie, Virus TT
Mots-clés Pascal anglais : Viral hepatitis, Viral disease, Infection, Blood plasma, Blood, Blood product, Prevalence, Statistical study, Molecular biology, Detection, Transmission from man to man, Risk factor, Human, Italy, Europe, Digestive diseases, Hepatic disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0504202
Code Inist : 002B05C02G. Création : 22/03/2000.