Association between coffee drinking and K-ras mutations in exocrine pancreatic cancer.
Study objective-To analyse the relation between coffee consumption and mutations in the K-ras gene in exocrine pancreatic cancer.
Consumption of coffee among cases with the activating mutation in the K-ras gene was compared with that of cases without the mutation.
Setting and patients-All cases of pancreatic cancer newly diagnosed at five hospitals in Spain during three years were included in the PANKRAS II Study (n=185, of whom 121 whose tissue was available for molecular analysis are the object of the present report).
Over 88% were personally interviewed in hospital.
DNA was amplified from paraffin wax embedded tissues, and mutations in codon 12 of K-ras were detected by the artificial RFLP technique.
Main results-Mutations were found in tumours from 94 of 121 patients (77.7%). Mutations were more common among regular coffee drinkers than among non-regular coffee drinkers (82.0% v 55.6%, p=0.018, n=107).
The odds ratio adjusted by age, sex, smoking and alcohol drinking was 5.41 (95% CI 1.64,17.78).
The weekly intake of coffee was significantly higher among patients with a mutated tumour (mean of 14.5 cups/week v 8.8 among patients with a wild type tumour, p<0.05).
With respect to non-regular coffee drinkers, the odds ratio of a mutated tumour adjusted by age, sex, smoking and alcohol drinking was 3.26 for drinkers of 2-7 cups/week, 5.77 for drinkers of 8-14 cups/week and 9.99 for drinkers of >=15 cups/week (p<0.01, test for trend). (...)
Mots-clés Pascal : Café, Consommation, Mutation, Gène, Tumeur maligne, Pancréas exocrine, Génétique, Epidémiologie, Facteur risque, Homme, Espagne, Europe, Alimentation, Appareil digestif pathologie, Pancréas pathologie, Gène K-ras
Mots-clés Pascal anglais : Coffee, Consumption, Mutation, Gene, Malignant tumor, Exocrine pancreas, Genetics, Epidemiology, Risk factor, Human, Spain, Europe, Feeding, Digestive diseases, Pancreatic disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0503454
Code Inist : 002B13C01. Création : 22/03/2000.