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  1. Detection of viral pathogens by reverse transcriptase PCR and of microbial indicators by standard methods in the canals of the Florida Keys.

    Article - En anglais

    In order to assess the microbial water quality in canal waters throughout the Florida Keys, a survey was conducted to determine the concentration of microbial fecal indicators and the presence of human pathogenic microorganisms.

    A total of 19 sites, including 17 canal sites and 2 nearshore water sites, were assayed for total coliforms, fecal coliforms, Escherichia coli, Clostridium perfringens, enterococci, coliphages, F-specific (F+) RNA coliphages, Giardia lamblia, Cryptosporidium parvum, and human enteric viruses (polioviruses, coxsackie A and B viruses, echoviruses, hepatitis A viruses, Norwalk viruses, and small round-structured viruses).

    Numbers of coliforms ranged from<1 to 1,410, E. coli organisms from<1 to 130, Clostridium spp. from<1 to 520, and enterococci from<1 to 800 CFU/100 ml of sample.

    Two sites were positive for coliphages, but no F+phages were identified.

    The sites were ranked according to microbial water quality and compared to various water quality standards and guidelines.

    Seventy-nine percent of the sites were positive for the presence of enteroviruses by reverse transcriptase PCR (polioviruses, coxsackie A and B viruses, and echoviruses).

    Sixty-three percent of the sites were positive for the presence of hepatitis A viruses.

    Ten percent of the sites were positive for the presence of Norwalk viruses.

    Ninety-five percent of the sites were positive for at least one of the virus groups. (...)

    Mots-clés Pascal : Floride, Etats Unis, Amérique du Nord, Amérique, Milieu marin, Pollution eau, Indicateur biologique, Fèces, Pathogène, Réaction chaîne polymérase, Canal, Bactérie, Bactériophage, Virus, Transcription inverse, Contamination biologique

    Mots-clés Pascal anglais : Florida, United States, North America, America, Marine environment, Water pollution, Biological indicator, Feces, Pathogenic, Polymerase chain reaction, Canal, Bacteria, Phage, Virus, Reverse transcription, Biological contamination

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0501690

    Code Inist : 002A05G. Création : 22/03/2000.