Detection of viral pathogens by reverse transcriptase PCR and of microbial indicators by standard methods in the canals of the Florida Keys.
In order to assess the microbial water quality in canal waters throughout the Florida Keys, a survey was conducted to determine the concentration of microbial fecal indicators and the presence of human pathogenic microorganisms.
A total of 19 sites, including 17 canal sites and 2 nearshore water sites, were assayed for total coliforms, fecal coliforms, Escherichia coli, Clostridium perfringens, enterococci, coliphages, F-specific (F+) RNA coliphages, Giardia lamblia, Cryptosporidium parvum, and human enteric viruses (polioviruses, coxsackie A and B viruses, echoviruses, hepatitis A viruses, Norwalk viruses, and small round-structured viruses).
Numbers of coliforms ranged from<1 to 1,410, E. coli organisms from<1 to 130, Clostridium spp. from<1 to 520, and enterococci from<1 to 800 CFU/100 ml of sample.
Two sites were positive for coliphages, but no F+phages were identified.
The sites were ranked according to microbial water quality and compared to various water quality standards and guidelines.
Seventy-nine percent of the sites were positive for the presence of enteroviruses by reverse transcriptase PCR (polioviruses, coxsackie A and B viruses, and echoviruses).
Sixty-three percent of the sites were positive for the presence of hepatitis A viruses.
Ten percent of the sites were positive for the presence of Norwalk viruses.
Ninety-five percent of the sites were positive for at least one of the virus groups. (...)
Mots-clés Pascal : Floride, Etats Unis, Amérique du Nord, Amérique, Milieu marin, Pollution eau, Indicateur biologique, Fèces, Pathogène, Réaction chaîne polymérase, Canal, Bactérie, Bactériophage, Virus, Transcription inverse, Contamination biologique
Mots-clés Pascal anglais : Florida, United States, North America, America, Marine environment, Water pollution, Biological indicator, Feces, Pathogenic, Polymerase chain reaction, Canal, Bacteria, Phage, Virus, Reverse transcription, Biological contamination
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0501690
Code Inist : 002A05G. Création : 22/03/2000.