A quantitative PCR-detection method was used to determine the presence of C. botulinum type B and E spores in eighty-seven fish representing six different species from two fish farms and ten wild freshwater whitefish.
Thirty percent (29/97) of the fish were positive for C. botulinum type E with no serotype B spores being detected.
The prevalence of type E in individual fish species varied from 4% to 100%. Fifteen type E isolates from the PCR-positive samples from one of the fish farms were characterized by pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA analysis and biochemical tests.
Five different genotypes were observed, with 11 of the isolates representing a predominant clone.
The discriminatory power of PFGE was superior to that of RAPD and biochemical tests.
Based on these results, it was concluded that a survey of the prevalence of type E in German fishery products is indicated in order to determine the public health risk.
Mots-clés Pascal : Poisson comestible, Contamination biologique, Pathogène, Détection, Réaction chaîne polymérase, Prévalence, Isolat, Typage, Génotype, Bavière, Allemagne, Europe, Clostridium botulinum, Clostridiaceae, Clostridiales, Bactérie
Mots-clés Pascal anglais : Edible fish, Biological contamination, Pathogenic, Detection, Polymerase chain reaction, Prevalence, Isolate, Typing, Genotype, Bavaria, Germany, Europe, Clostridium botulinum, Clostridiaceae, Clostridiales, Bacteria
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0491615
Code Inist : 002A35B06. Création : 22/03/2000.