Hepatitis C Virus (HCV) detection in the livers of chronically infected patients remains a debatable issue.
To determine the significance of hepatic expression of hepatitis C viral antigen c100, an immunohistochemical assay was performed in 113 young thalassemics with chronic HCV infection.
One hundred and thirteen patients were seropositive for antibody to HCV by second-generation testing.
The monoclonal antibody TORDJI-22 was used in an alkaline phosphatase 3-step staining method, and any possible association between the results of HCV immunodetection and various clinicopathologic variables was investigated by univariate and multivariate statistical analysis.
In 36 cases, post-therapy liver biopsy specimens were also studied.
HCV c100 antigen was detected in 62% of all pretherapy samples, exclusively in the cytoplasm of rather few hepatocytes.
Its expression was positively associated with male gender (p=0.02) as well as with rather advanced age (p=0.03) and was frequently accompanied by low necroinflammatory scores (according to the modified HAI grading).
At the end of interferon-alpha (IFN-alpha) therapy, the immunoreactive prevalence of c100 antigen decreased significantly (pF=0.002).
We conclude that hepatic expression of c100 antigen is detected in a considerable percentage of thalassemics but it is not likely to provide information concerning the viral load in the infected liver. (...)
Mots-clés Pascal : Thalassémie, Dépistage, Association morbide, Hépatite virale C, Virose, Infection, Antigène, Virus hépatite C, Hepacivirus, Flaviviridae, Virus, Immunohistochimie, Epidémiologie, Homme, Hémopathie, Anémie hémolytique, Hémoglobinopathie, Maladie héréditaire, Appareil digestif pathologie, Foie pathologie, Anatomopathologie
Mots-clés Pascal anglais : Thalassemia, Medical screening, Concomitant disease, Viral hepatitis C, Viral disease, Infection, Antigen, Hepatitis C virus, Hepacivirus, Flaviviridae, Virus, Immunohistochemistry, Epidemiology, Human, Hemopathy, Hemolytic anemia, Hemoglobinopathy, Genetic disease, Digestive diseases, Hepatic disease, Pathology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0482088
Code Inist : 002B22D04. Création : 22/03/2000.