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  1. Strategies for automated sample preparation, nucleic acid purification, and concentration of low target number nucleic acids in environmental and food processing samples.

    Article, Communication - En anglais

    Pathogen detection and remediation for safe eating. Conference. Boston, MA, USA, 1998/11/05.

    The purpose of this work is to develop a rapid, automated system for nucleic acid purification and concentration from environmental and food processing samples.

    Our current approach involves off-line filtration and cell lysis (ballistic disintegration) functions in appropriate buffers followed by automated nucleic acid capture and purification on renewable affinity matrix microcolumns.

    Physical cell lysis and renewable affinity microcolumns eliminate the need for toxic organic solvents, enzyme digestions or other time-consuming sample manipulations.

    Within the renewable affinity microcolumn, we have examined nucleic acid capture and purification efficiency with various microbead matrices (glass, polymer, paramagnetic), surface derivatization (sequence-specific capture oligonucleotides or peptide nucleic acids), and DNA target size and concentration under variable solution conditions and temperatures.

    Results will be presented comparing automated system performance relative to benchtop procedures for both clean (pure DNA from a laboratory culture) and environmental (soil extract) samples, including results which demonstrate 8 minute purification and elution of low-copy nucleic acid targets from a crude soil extract in a form suitable for PCR or microarray-based detectors. (...)

    Mots-clés Pascal : Analyse automatique, Concentration chimique, Détection, Hybridation moléculaire, Injection en écoulement, Préparation échantillon, Purification, Stratégie, Traitement automatisé, Environnement, Air, Eau, Sol, Produit alimentaire, Acide nucléique, DNA, Microorganisme, Pathogène, Analyse biochimique, Bactériologie, Biologie moléculaire, Méthode analytique, Microbiologie appliquée

    Mots-clés Pascal anglais : Automatic analysis, Chemical concentration, Detection, Molecular hybridization, Flow injection, Sample preparation, Purification, Strategy, Automated processing, Environment, Air, Water, Soils, Foodstuff, Nucleic acid, DNA, Microorganism, Pathogenic, Biochemical analysis, Bacteriology, Molecular biology, Analytical method, Applied microbiology

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0481875

    Code Inist : 002A05B14. Création : 22/03/2000.