To investigate cellular immune responses in laboratory animal workers who are exposed to high levels of animal allergens but also to other biologically active substances containing lipopolysaccharides (LPS), i.e., endotoxins.
A survey among 20 animal facility workers and 20 matched (gender, smoking) controls was conducted using exposure measurements (endotoxin, colony-forming units of bacteria and fungi) and a questionnaire on respiratory symptoms.
Blood samples were taken to determine the ex vivo whole-blood release of tumor necrosis factor-alpha (TNF) and interleukin-8 (IL-8) as well as plasma levels of LPS-binding protein (LBP), bactericidal permeability-increasing protein (BPI), the 75-kDa soluble TNF receptor (sTNFR75), and total/specific IgE.
Although exposure to endotoxin was low (range 0.05-2.8 ng/m3), a significant (P<0.05) increase in plasma BPI (4-fold) and srTNF75 (1.2-fold) was found, suggesting a response to inhalation of LPS.
Also, the capacity of blood leukocytes to release TNF and IL-8 in response to ex vivo exposure to workplace dust was increased.
Data were not confounded by specific allergies, levels of IgE, smoking, or respiratory symptoms.
A profound effect on the cellular immune response was seen in animal workers with low endotoxin exposure and a high antigenic load.
It remains to be determined which other biologically active substance (s) are involved in this effect.
Mots-clés Pascal : Animalerie, Animal laboratoire, Exposition professionnelle, Médecine travail, Homme, Allergie, Réponse immune, Lipopolyoside, Endotoxine, Toxicité, Protéine liaison, IgE, Immunopathologie
Mots-clés Pascal anglais : Laboratory animal facility, Laboratory animal, Occupational exposure, Occupational medicine, Human, Allergy, Immune response, Lipopolysaccharide, Endotoxin, Toxicity, Binding protein, IgE, Immunopathology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0474507
Code Inist : 002B06C04. Création : 22/03/2000.