In this study, the role of delta-aminolevulinic acid dehydratase (ALAD) variants in lead susceptibility was examined.
The study subjects comprised 223 male workers, and the relationship between their blood lead level and erythrocyte ALAD activity or plasma/urine delta-aminole-vulinic acid level was studied.
Leukocyte specimens from 11 workers, whose erythrocyte ALAD activities were as low as one-fifth that of the other normal workers, were subjected to analyses of their ALAD1 and ALAD2 alleles.
Further, the entire exon fragment of the ALAD gene was analyzed by polymerase chain reaction, and the reaction product was used as a target for direct DNA sequencing.
Genomic DNA analysis revealed that all 11 workers had the ALAD1-1 allele, whereas the entire ALAD gene analysis failed to indicate other variants, except for the Rsa I site.
The depletion in erythrocyte ALAD activity was not found to be caused by the ALAD2 allele.
Mots-clés Pascal : Plomb, Métal lourd, Toxicité, Homme, Exposition professionnelle, Médecine travail, Sensibilité résistance, Porphobilinogen synthase, Hydro-lyases, Carbon-oxygen lyases, Lyases, Enzyme, Génotype, Prédisposition, Polymorphisme, Génétique, Plasma sanguin, Urine, Liquide biologique, Allèle
Mots-clés Pascal anglais : Lead, Heavy metal, Toxicity, Human, Occupational exposure, Occupational medicine, Sensitivity resistance, Porphobilinogen synthase, Hydro-lyases, Carbon-oxygen lyases, Lyases, Enzyme, Genotype, Predisposition, Polymorphism, Genetics, Blood plasma, Urine, Biological fluid, Allele
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0460210
Code Inist : 002B03L05. Création : 22/03/2000.