A specific and sensitive method based on gas chromatography/tandem mass spectrometry with on-column injection was developed to quantify simultaneously cyclophosphamide and ifosphamide in urine by using trophosphamide as an internal standard.
The urine samples were extracted with diethyl ether and derivatization was performed with heptafluorobutyric anhydride.
The detection limits of cyclophosphamide and ifosphamide in urine samples were 0.1 and 0.5 ng ml-1, respectively, with a signal-to-noise ratio of 3 : 1. The sensitivity, the specificity and the low cost of the instrumentation involved make this method suitable for economical analysis on a large scale, such as for the biological monitoring of occupational exposure to cyclophosphamide and ifosphamide in production plants and in hospitals during their pharmacological use.
Mots-clés Pascal : Analyse chimique, Analyse quantitative, Cyclophosphamide, Ifosfamide, Hétérocycle oxygène azote phosphore, Oxazaphosphinane dérivé, Phosphorodiamidate organique, Phosphore Composé organique, Anticancéreux, Agent alkylant, Moutarde à l'azote, Carcinogène, Liquide biologique, Urine, Analyse trace, Chromatographie phase gazeuse, Méthode couplée, Spectrométrie masse tandem, Impact électron, Préparation échantillon, Extraction liquide liquide, Extraction solvant, Dérivatisation, Surveillance biologique, Exposition professionnelle, Industrie pharmaceutique, Service hospitalier, Homme, Médecine travail, Butyrique acide(perfluoro) anhydride
Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Cyclophosphamide, Ifosfamide, Oxygen nitrogen phosphorus heterocycle, Oxazaphosphinane derivatives, Organic phosphorodiamidate, Phosphorus Organic compounds, Antineoplastic agent, Alkylating agent, Nitrogen mustard, Carcinogen, Biological fluid, Urine, Trace analysis, Gas chromatography, Coupled method, Mass spectrometry MS/MS, Electron impact, Sample preparation, Liquid liquid extraction, Solvent extraction, Derivatization, Biological monitoring, Occupational exposure, Pharmaceutical industry, Hospital ward, Human, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0456832
Code Inist : 002B02A02. Création : 22/03/2000.