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  1. Effectiveness of a manual disinfection procedure in eliminating hepatitis C virus from experimentally contaminated endoscopes.

    Article, Communication - En anglais

    Annual Meeting of the Society for Healthcare Epidemiology of America. Orlando, FL, USA, 1998/04.


    Transmission of hepatitis C virus (HCV) through endoscopy has been reported, but the Implications as a public health concern remain controversial.

    This study investigated the degree to which a thorough manual cleaning-washing-disinfectio procedure can decontaminate all channels of a flexible submersible endoscope experimentally contaminated with HCV.


    To assess the accuracy of the method currently in use, the initial investigation focused on sampling effectiveness.

    Nine endoscopes were contaminated with high-titer HCV-positive plasma and flushed with 150 mL of sampling solution (distilled water) before disinfection.

    To assess the effectiveness of the disinfection procedure, the following sequence was performed on another 10 endoscopes : inoculation, disinfection, and sampling.

    After concentration residual viruses were detected by means of RNA amplification with commercial assays.


    The study showed that sampling alone can reduce viral titer to one-fourth its original value.

    Within the limits of this method, HCV RNA was never detected by means of polymerase chain reaction after disinfection, whereas all internal amplification controls were positive.

    This reduction to less than 1/100,000 of original titer exceeds the criterion expected for the virucidal activity of disinfectants. (...)

    Mots-clés Pascal : Hépatite virale C, Virose, Infection, Etude expérimentale, In vitro, Endoscope, Contamination, Procédure, Désinfection, Manuel, Transmission homme homme, Efficacité, Evaluation, Homme, Appareil digestif pathologie, Foie pathologie, Matériau, Instrument, Hygiène

    Mots-clés Pascal anglais : Viral hepatitis C, Viral disease, Infection, Experimental study, In vitro, Endoscope, Contamination, Procedure, Disinfection, Manuals, Transmission from man to man, Efficiency, Evaluation, Human, Digestive diseases, Hepatic disease, Materials, Instrument, Hygiene

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0455452

    Code Inist : 002B24E08. Création : 22/03/2000.