To assess individual exposure to monoaromatic hydrocarbons (benzene, toluene, ethylbenzene and xylenes - BTEX) in biological fluids, a GC-MS method was developed.
Headspace sampling of BTEX was by solid-phase microextraction (SPME) with a 75 mum Carboxenpolydimethylsiloxane (PDMS) fiber.
Linearity was established for concentrations up to 50 mug L-1.
Detection limits, calculated both in human blood and urine, ranged 5-10ng L-1.
Repeatability was in the range 6.5-9.2% for all compounds.
The method was applied to the evaluation of the internal dose of BTEX in a group of cyclists running for 2 h within city routes.
Benzene and toluene in blood, and toluene and xylenes in urine significantly increased after exercise as compared to prerun values, such changes being consistent with airborne concentrations.
The combination of SPME with GC-MS seems to represent an appropriate analytical approach to detect changes in the concentration of monoaromatic hydrocarbons in biological media resulting from exposure to environmental pollution.
Mots-clés Pascal : Analyse chimique, Analyse quantitative, Analyse qualitative, Hydrocarbure, Benzène, Toluène, Xylène, Polluant, Liquide biologique, Sang, Urine, Homme, Surveillance biologique, Sportif, Cyclisme, Zone urbaine, Préparation échantillon, Extraction SPE, Espace tête, Chromatographie phase gazeuse, Méthode couplée, Spectrométrie masse, Benzène(éthyl), BTEX
Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Qualitative analysis, Hydrocarbon, Benzene, Toluene, Xylene, Pollutant, Biological fluid, Blood, Urine, Human, Biological monitoring, Athlete, Cyclism, Urban area, Sample preparation, Solid phase extraction, Headspace, Gas chromatography, Coupled method, Mass spectrometry
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0455057
Code Inist : 002B03L06. Création : 22/03/2000.