Isocyanates are a group of highly reactive cross-linking chemicals that cause airway inflammation and asthma in exposed individuals.
Isocyanates have been detected along the airway epithelia of exposed workers and animals, prompting the hypothesis that isocyanates can directly bind to epithelial cell proteins.
We tested the hypothesis that hexamethylene diisocyanate (HDI) binds directly to lung epithelial cell proteins and initiated studies to evaluate the immunostimulatory potential of HDI-conjugated lung epithelial cell proteins.
Human lung epithelial cell lines were exposed to vapor-and liquid-phase HDI, and the cellular proteins were analyzed for HDI conjugation by Western blotting and tested for the ability to induce lymphocyte proliferation in vitro.
A number of epithelial cell polypeptides, ranging from 25 to 110 kd in apparent molecular weight, were conjugated with HDI after exposure of the human lung epithelial cell lines (A549 and NCI-H292) to HDI concentrations greater than 0.005% (vol/vol) in the liquid phase.
Vapor-phase HDI exposure resulted in a more restricted HDI conjugation pattern, with major HDI-conjugated polypeptides migrating at 47,71, and 91 kd.
HDI-conjugated epithelial cell proteins specifically stimulated proliferation of PBMCs from subjects with isocyanate-induced asthma but not HDI-exposed nonasthmatic individuals or atopic subjects with nonisocyanate-related asthma. (...)
Mots-clés Pascal : Asthme, Isocyanate organique, Exposition professionnelle, Pathogénie, Poumon, Cellule épithéliale, Protéine, Inflammation, Lymphocyte, Conjugué haptène protéine, Réponse immune, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Médecine travail
Mots-clés Pascal anglais : Asthma, Organic isocyanate, Occupational exposure, Pathogenesis, Lung, Epithelial cell, Protein, Inflammation, Lymphocyte, Hapten protein conjugate, Immune response, Human, Respiratory disease, Obstructive pulmonary disease, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0454962
Code Inist : 002B11B. Création : 22/03/2000.