We tried to test the diagnostic efficacy of a new 13C-labeled urea agent made in Taiwan for urea breath test (UBT) of H. pylori infection, and to assess the correlation between the bacterial load of H. pylori in the stomach and the results of UBT from different timings.
One hundred and ninety-six dyspeptic patients without usage of antibiotics and proton pump inhibitors in the last 4 weeks were recruited for endoscopy, which included CLO test and H. pylori culture.
Three additional bits of gastric biopsy (each one from antrum, body, and cardia) were taken for histology to assess the H. pylori density (HPD, range 0-5) in each specimen and the total bacterial density (TBD, a sum of HPD from three sites, range 0-15).
Every study patient had been assigned to complete the UBT protocol.
The gas samplings of UBT at baseline, 15min and 30min after ingestion of 100mg 13C-labeled urea (INER-Hp 13C-tester, Taiwan) were collected for the ratio of 13 CO2/12CO2 and labeled A, B, and C respectively.
Both A15 (B minus A) & A30 (C minus A) were recorded to express the excess delta13CO2 per milliliter.
During the 30min period of UBT, the patient was scheduled to change lying positions every 5 minutes for even coating of the stomach with test agent.
Based on two positive results of three invasive methods (CLO test, culture, and histology), 91 cases were confirmed to have H. pylori infection.
The diagnostic efficacy of UBT was quite good with 96. (...)
Mots-clés Pascal : Helicobacter pylori, Spirillaceae, Spirillales, Bactérie, Estomac, Diagnostic, Test respiratoire, Urée, Marqueur biologique, Densité charge, Analyse quantitative, Corrélation, Homme, Taiwan, Asie, Bactériose, Infection, Appareil digestif pathologie, Estomac pathologie, Traitement instrumental
Mots-clés Pascal anglais : Helicobacter pylori, Spirillaceae, Spirillales, Bacteria, Stomach, Diagnosis, Breath test, Urea, Biological marker, Charge density, Quantitative analysis, Correlation, Human, Taiwan, Asia, Bacteriosis, Infection, Digestive diseases, Gastric disease, Instrumentation therapy
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0444827
Code Inist : 002B24O06. Création : 22/03/2000.