The aim of this study was to describe a detailed and validated methodology designed for the analysis of carcinogenic N-nitrosodiethanolamine (NDELA) down to sub-mug/l levels in urine and its application to a number of workers exposed to NDELA-contaminated aqueous metalworking fluids (MWF).
Following a work-up procedure based on solid-phase extraction of NDELA, the urinary extracts were analysed without derivatization by gas chromatography on a polar wide-bore column with chemiluminescent detection using a thermal energy analyser (TEA).
N-Nitroso- (2-hydroxypropyl) amine was used as an internal standard.
The method was applied to 12 workers using « nitrite-free » or « nitrite-formulated » MWF and to 15 unexposed subjects.
The NDELA content of the MWF was also determined using a similar, but simpler method able to easily quantify NDELA down to at least 0.1 mg/l. Results : Contamination by NDELA traces of some chemicals used for the sample preparation, particularly ethyl formate, must be carefully checked since it can give rise to false-positive results of up to I or 2 mug/l. The response was linear in the range of 0-500 mug/l. Between 0.5 and 10 mug/l, the recovery rate was close to 95%, while repeatability ranged from 12.5 to 6.4% (n=5).
The detection limit was 0.3 mug/l (Signal/noise=3).
No detectable NDELA could be observed in the control workers. (...)
Mots-clés Pascal : Fluide travail, Fluide coupe, Huile travail métal, Nitrosamine, Carcinogène, Toxicité, Exposition professionnelle, Médecine travail, Homme, Surveillance biologique, Marqueur biologique, Urine, Liquide biologique, Analyse quantitative, Industrie métallurgique, Chromatographie phase gazeuse, Energie thermique, Diethanolamine(N-nitroso)
Mots-clés Pascal anglais : Working fluid, Cutting fluid, Metalworking oil, Nitrosamine, Carcinogen, Toxicity, Occupational exposure, Occupational medicine, Human, Biological monitoring, Biological marker, Urine, Biological fluid, Quantitative analysis, Metallurgical industry, Gas chromatography, Thermal energy
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0437710
Code Inist : 002B03L06. Création : 22/03/2000.