Influence of GSTM1 genotypes on anti-BPDE-DNA adduct levels in mononuclear white blood cells of humans exposed to PAH.
Association between genetic deletion polymorphism of GSTMI (*0/*0 or active) and levels of anti (±) - r-7, t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti-BPDE) - DNA adducts in the peripheral blood lymphocyte plus monocyte fraction (LMF) of PAH-exposed subjects was investigated.
A total of 94 Caucasian subjects comprised the sample population : 13 coke-oven workers, 19 chimney sweeps, 36 aluminum-anode plant workers, and 26 non-occupationally PAH-exposed subjects (controls).
PAH exposure was assessed in each group by means of the urinary excretion of 1-pyrenol (mean group levels 1.2,0.7,0.3, and 0.1 mumol/mol creatinine in coke-oven workers, chimney sweeps, aluminum-anode plant workers, and control subjects, respectively).
Anti-BPDE-DNA adducts were detected by HPLC/fluorescence analysis of anti-BPDE tetrols (tetrol I-1) released after acid hydrolysis of DNA samples.
In coke-oven workers the percentage of cases with adduct levels exceeding the 95th percentile control value (4.4 adducts/108 nucleotides) was significantly higher in the subgroup with the null GSTMI genotype (*0/*0) (100%) than in that with active GSTMI (43% ; X2 test, P<0.05).
In the other groups with different and lower levels of PAH exposure the percentages of positive samples were always higher in the subgroup with GSTMI *0/*0 than in the active one, although the differences were not statistically significant. (...)
Mots-clés Pascal : Composé aromatique polycyclique, Homme, Exposition professionnelle, Médecine travail, Polymorphisme, Génétique, Génotype, Glutathione transferase, Transferases, Enzyme, Adduit moléculaire, Benzo[a]pyrène-7,8-diol(9,10-époxy-7,8,9,10-tétrahydro), Leucocyte, Mononucléaire, Surveillance biologique, Prédisposition, Toxicité, Gène GSTM1
Mots-clés Pascal anglais : Polycyclic aromatic compound, Human, Occupational exposure, Occupational medicine, Polymorphism, Genetics, Genotype, Glutathione transferase, Transferases, Enzyme, Molecular adduct, Leukocyte, Mononuclear cell, Biological monitoring, Predisposition, Toxicity
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0436302
Code Inist : 002B03L06. Création : 22/03/2000.