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  1. Biological monitoring of workers exposed to 4,4-methylene-bis- (2-orthochloroaniline) (MOCA). I. A new and easy determination of "free" and "total" MOCA in urine.

    Article - En anglais


    The objective of the study was to validate a new and simple method to determine MOCA in the urine of exposed workers.


    The separation, identification and quantification of urinary MOCA were performed in spiked urines by a sensitive and practical high-performance liquid chromatography (HPLC) method and applied to urine samples of 11 workers occupationally exposed to MOCA ; the postshift urinary levels of MOCA in their urine samples with and without hydrolysis, « total » and « free » MOCA respectively, were determined.

    In addition, we investigated the use of citric or sulfamic acid as preservatives of urine samples.


    The « total » and « free » MOCA were extracted with isooctane from hydrolysed and nonhydrolysed 20-ml urine samples respectively.

    After evaporation, the residue was dissolved in 4 ml of 2. 10-2 M aqueous hydrochloric acid and analysed by an isocratic HPLC system using both ultraviolet (UV) detection at 244 nm and electrochemical detection working in oxidation mode (0.9 V) with an Ag/AgCl reference electrode.

    Mobile phase (50% acetonitrile in water containing 0.4% acetate buffer solution pH=4.6) was used to complete the 20-min analysis. « Free » and « total » MOCA were chromatographed on a reversed-phase C8 column (5 mum ; 250 mm x 4 mm).

    The standard curve of MOCA was linear over the range 5-500 mug/l in human urine.

    The detection limit was 1 mug/l for a 20-mul injection volume ; the repeatability ranged from 5.6 to 1. (...)

    Mots-clés Pascal : Amine, Composé aromatique, Exposition professionnelle, Médecine travail, Homme, Surveillance biologique, Urine, Liquide biologique, Analyse quantitative, Chromatographie HPLC, Analyse électrochimique, 4,4p-Méthylènedianiline(2,2p-dichloro)

    Mots-clés Pascal anglais : Amine, Aromatic compound, Occupational exposure, Occupational medicine, Human, Biological monitoring, Urine, Biological fluid, Quantitative analysis, HPLC chromatography, Electrochemical analysis

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0436299

    Code Inist : 002B03L06. Création : 22/03/2000.