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  1. High-performance liquid chromatographic determination of n-methylformamide, a biological index for occupational exposure to dimethylformamide.

    Article, Communication - En anglais

    ISC'98 International Symposium on Chromatography. Rome, ITA, 1998/09/13.

    This report describes an analytical method for the biological monitoring of workers exposed to N, N-dimethylformamide (DMF), a solvent widely used in the chemical industry.

    The human main metabolites of DMF are N-hydroxymethyl-N-methylformamide (HMMF) and the minor metabolites N-methylformamide (NMF) and N-acetyl-S- (N-methylcarbamoyl) cysteine.

    The metabolite selected by the American Conference of Governmental Hygienists for occupational biomonitoring purposes, is NMF measured by gas chromatographic analysis, as during it HMMF may be converted to the minor metabolite NMF.

    HMMF and NFM can be measured independently using HPLC analysis.

    The procedure proposed here involves the thermal transformation of the primary metabolite HMMF into the minor metabolite NMF, which is then determined by HPLC.

    This method makes it possible to determine, using HPLC, both metabolites of DMF by measuring only one peak, thus offering two major advantages : (i) it increases the sensitivity of the test and (ii) it deploys only one reference standard.

    Mots-clés Pascal : Analyse chimique, Analyse quantitative, Métabolite, DMF, Solvant organique, Surveillance biologique, Exposition professionnelle, Industrie chimique, Médecine travail, Carboxamide, Liquide biologique, Urine, Homme, Chromatographie HPLC, Détecteur UV, Préparation échantillon, Extraction SPE, Réaction thermique, Formique acide méthylamide, Formique acide [hydroxyméthyl méthyl]amide

    Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Metabolite, N,N-Dimethylformamide, Organic solvent, Biological monitoring, Occupational exposure, Chemical industry, Occupational medicine, Carboxamide, Biological fluid, Urine, Human, HPLC chromatography, Ultraviolet detector, Sample preparation, Solid phase extraction, Thermal reaction

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0420938

    Code Inist : 002B03L04. Création : 22/03/2000.