logo BDSP

Base documentaire

  1. Determination of naphthalene metabolites in human urine by liquid chromatography-mass spectrometry with electrospray ionization.

    Article, Communication - En anglais

    ISC'98 International Symposium on Chromatography. Rome, ITA, 1998/09/13.

    The use of a liquid chromatography-electrospray mass spectrometry system was investigated for the quantitative analysis of naphthalene metabolites (alpha-naphthol, alpha-naphthylglucuronide and bêta-naphthylsulphate) in untreated urine samples.

    Chromatography was carried out under ion-suppressed reversed-phase conditions, by using high-speed (3 cm, 3 mum) columns and formic acid (2 mM) as a modifier in the mobile phase.

    The ionization was obtained in the negative-ion mode.

    Linearity, sensitivity and precision of the method were explored by operating in selected-ion monitoring mode.

    The method was applied to the quantitative analysis of naphthalene metabolites in untreated urine samples from workers in a naphthalene producing plant.

    Solid-phase extraction was used for sample clean-up and trace enrichment.

    Liquid chromatography-tandem mass spectrometry experiments were performed for confirmation purposes.

    Mots-clés Pascal : Analyse chimique, Analyse quantitative, Analyse qualitative, Métabolite, Composé aromatique polycyclique, Naphtalène dérivé, Naphtol, Glucuroconjugué, Sulfate organique, Liquide biologique, Urine, Homme, Surveillance biologique, Exposition professionnelle, Médecine travail, Chromatographie phase inverse, Chromatographie HPLC, Méthode couplée, Spectrométrie masse, Electrospray, Spectrométrie masse tandem, Préparation échantillon, Enrichissement chimique, Extraction SPE

    Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Qualitative analysis, Metabolite, Polycyclic aromatic compound, Naphthalene derivatives, Naphthol, Glucuroconjugate, Organic sulfate, Biological fluid, Urine, Human, Biological monitoring, Occupational exposure, Occupational medicine, Reversed phase chromatography, HPLC chromatography, Coupled method, Mass spectrometry, Electrospray, Mass spectrometry MS/MS, Sample preparation, Chemical enrichment, Solid phase extraction

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0420937

    Code Inist : 002B03L06. Création : 22/03/2000.