Workers in epoxy resin, synthetic leather, and printed circuit board manufacturing plants are exposed to epichlorohydrin (ECH), or dimethylformamide (DMF), or both.
ECH, an alkylating agent, has been shown to cause malignancy in animals, but its genotoxicity in humans is unclear.
DMF is a well-known hepatotoxic chemical, although evidence of its genotoxicity in humans is also limited.
In this study, we examined the effects of exposure to ECH and DMF on sister chromatid exchange (SCE) in plant workers, in order to examine the genotoxicity of these two agents.
Because the genotoxicity of certain agents can be modulated by metabolic traits, we also investigated influence of the glutathione S-transferase (GST) mu (GST M1) and GST 0 (GST T1) genes on the genotoxicity of ECH and DMF.
A total of 85 male plant workers were included in this study.
The subjects were divided into five exposure groups, based on their job titles and the airborne ECH and DMF concentrations in their areas of work.
A questionnaire was administered to obtain detailed occupational, smoking, alcohol consumption, and medication histories.
Standardized cytogenetic methods were used to determine the frequency of sister chromatid exchange (SCE) in peripheral blood lymphocytes.
GST Ml and GST Tl genotypes were identified using polymerase chain reaction (PCR).
In analysis, smoking was significantly associated with increased SCE frequency (P<0.01). (...)
Mots-clés Pascal : Oxirane(chlorométhyl), DMF, Toxicité, Solvant organique, DNA, Test mutagénicité, Echange chromatide soeur, Homme, Exposition professionnelle, Médecine travail, Lymphocyte, Glutathione transferase, Transferases, Enzyme
Mots-clés Pascal anglais : Epichlorhydrin, N,N-Dimethylformamide, Toxicity, Organic solvent, DNA, Mutagenicity testing, Sister chromatid exchange, Human, Occupational exposure, Occupational medicine, Lymphocyte, Glutathione transferase, Transferases, Enzyme
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0419073
Code Inist : 002B03L04. Création : 22/03/2000.