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  1. Ozone-induced lung function decrements do not correlate with early airway inflammatory or antioxidant responses.

    Article - En anglais

    Kelly, A.J. Frew, S.T. Holgate, T. Sandstrom.

    ERS Journals Ltd 1999.

    This study sought to clarify the early events occurring within the airways of healthy human subjects performing moderate intermittent exercise following ozone challenge.

    Thirteen healthy nonsmoking subjects were exposed in a single blinded, crossover control fashion to 0.2 parts per million (ppm) O3 and filtered air for 2 h, using a standard intermittent exercise and rest protocol.

    Lung function was assessed pre-and immediately post-exposure.

    Bronchoscopy was performed with endobronchial mucosal biopsies, bronchial wash (BW) and bronchoalveolar lavage (BAL) 1.5 h after the end of the exposure period.

    Respiratory tract lining fluid (RTLF) redox status was assessed by measuring a range of antioxidants and oxidative damage markers in BW and BAL fluid samples.

    There was a significant upregulation after O3 exposure in the expression of vascular endothelial P-selectin (p<0.005) and intercellular adhesion molecule-1 (p<0.005).

    This was associated with a 2-fold increase in submucosal mast cells (p<0.005) in biopsy samples, without evidence of neutrophilic inflammation, and a decrease in BAL fluid macrophage numbers (1.6-fold, p<0.005), with an activation of the remaining macrophage subset (2.5-fold increase in% human leukocyte antigen (HLA) - DR+cells, p<0.005).

    In addition, exposure led to a 4.5-fold and 3.1-fold increase of reduced glutathione (GSH) concentrations, in BW and BAL fluid respectively (p<0. (...)

    Mots-clés Pascal : Ozone, Homme, Epreuve effort, Exposition, Exploration, Fonction respiratoire, Bronchoscopie, Lavage bronchoalvéolaire, Stress oxydatif, Inflammation, Etude expérimentale, Appareil respiratoire, Endoscopie

    Mots-clés Pascal anglais : Ozone, Human, Exercise tolerance test, Exposure, Exploration, Lung function, Bronchoscopy, Bronchoalveolar lavage, Oxidative stress, Inflammation, Experimental study, Respiratory system, Endoscopy

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    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0414206

    Code Inist : 002B30A02A. Création : 22/03/2000.